Consultants & Laboratories

For additional information on testing and pricing call (703) 471-4200.

Why Test for Legionella pneumophila?

Three levels of turnaround time (TAT) are available.  Although handing times will vary depending on the test additional surcharges may apply for expedited services:



Routine          3-10 days         1 x routine price

Rush               2 days               2 x routine price

Emergency     1 day                3 x routine price

Microbiology Laboratory Services

Laboratory Director

Jonathon Hall

(703) 471-4200


Department of the Environment Water Supply Program


Certified Drinking Water Laboratory

Chain of Custody Forms

Center Disease Control and Prevention (CDC)

ELITE Program

Environmental Legionella Isolation Techniques Evaluation

Senior Microbiologist

Raymond Petrisek

(703) 471-4200

NOTE: Reference materials and links provided on our site are only intended to be used by clients of HPE who consult with us on these subjects.  External links do not imply any endorsement or support for any position, service or product.  Interpretive guidelines for laboratory results are only to be used by experienced and qualified experts in conjunction with all other necessary data and information.

Routine Environmental Testing Services

Microbiology Laboratory Overview

Quality Assurance

HP Environmental, Inc. considers the accuracy, reliability and reproducibility of analysis results to be a fundamental responsibility of laboratory management and is paramount to our continued effort to produce a superior product for our client.  

Our commitment and dedication to quality is fully supported and communicated from management to the bench level to meet the objectives of the laboratory and to effectively serve client requirements.  In order to achieve this goal, the laboratory has developed and executed a robust quality assurance program to control the precision and accuracy of data generated and limit sources of bias.  

The objective of the quality assurance program is to produce reliable data of measurable quality that is defensible, representative of the sample and meets all required State, Environmental Protection Agency (EPA), National Institute of Occupational Health (NIOSH), American Industrial Hygiene Associate (AIHA), American Society of Testing and Materials (ASTM) and data user requirements.   Complete adherence to laboratory standard operating procedures (SOPs), execution of good laboratory practices (GLPs), internal audit reviews and participation in external performance testing programs ensure the laboratory produces data of known and documented quality using EPA, AIHA, ASTM and NIOSH protocols and client specific requirements is mandated by management.

Contact us today to learn more about our microbiological testing services

(routine & customized methods) or to obtain a quote for your next PROJECT!

American Industrial Hygiene Association


Environmental Microbiology

Laboratory Accreditation Program





Order Containers, Media & Supplies

Online Test Request Form

HP Environmental, Inc. specializes in the testing of environmental water samples for Legionella and other pathogens at a competitive and reasonable cost.  Legionella testing is performed using the standard culture method, Legiolert (developed by IDEXX) and direct fluorescent antibody (DFA). Our microbiology laboratory is ACCREDITED by the NYS Department of Health Environmental (NYSDOH) and is proficient in the analysis of Legionella using the internationally recognized standard ISO 11731:2017(E). The laboratory is also accredited by the American Industrial Hygiene Services (AIHA-LAP, LLC) in environmental microbiology and is approved as a CERTIFIED Drinking Water Laboratory in Virginia (Certification # 00156) and Maryland (Certification # 319). The Microbiology Laboratory is a member of the CDC ELITE program and is a Sentinel Laboratory certified by the Commonwealth of Virginia, Department of Laboratory Services.

Our services also include the analysis of bacteria and fungi in air, water, wipe/swab, and bulk environmental samples. Refer to the tables below for a detailed list of testing services. If you have any questions about our services, pricing or need sample bottles please order online or contact our office at (703) 471-4200.

Click the logo above to upload a copy of our Accreditation or Member Certificate.

Test ProcedureMatrixMethodTAT
ISO 11731:2017WaterCulture (CFU/mL)7 - 10 days
Direct Fluorescence Antibody (DFA)WaterDirect Microscopy (P/A)2 days
L. pnuemophila (ONLY)WaterLegiolert (MPN/mL)7 days
New York State ProtocolWaterISO 11731 (CFU/mL)7 - 10 days
Test ProcedureMatrixMethodTAT
Acid Producing BacteriaWaterBART (HACH)10 days
Denitrifying BacteriaWaterBART (HACH)10 days
EnterococcusWaterDirect Substrate Technology (P/A)2 days
Environmental MycobacteriumWater, Wipe, Bulk, AirCulture2 - 6 weeks
Iron-Related BacteriaWaterBART (HACH)10 days
Nitrifying BacteriaWaterBART (HACH)10 days
Total Coliforms / E. coliWater, WipePotability (P/A)2 days
Total Coliforms / E. coliWaterPotability (MPN/mL)2 days
Total Plate Count (TPC)Water, Wipe, Bulk, AirCulture (w/ or w/o IDs)3 - 5 days
TPC (Anaerobic)Water, Wipe, Bulk, AirCulture (w/o IDs)3 - 5 days
Pseudomonas aeruginosaWaterDirect Substrate Technology (P/A)2 days
Slime-Forming BacteriaWaterBART (HACH)10 days

Legionella Analyses

Bacterial Analyses

Fungal Analyses

Test ProcedureMatrixMethodTAT
Direct MicroscopyBulk, WipeTape-Lift3 - 5 days
Total Fungal Count (TFC)Water, Wipe, Bulk, AirCulture (w/ or w/o IDs)5 - 7 days
Total Thermophilic Fungal CountWater, Wipe, Bulk, AirCulture5 - 7 days
Total Thermophilic Actinomycete CountWater, Wipe, Bulk, AirCulture5 - 7 days
Total Spore CountAirDirect Microscopy3 - 5 days

Other Analyses

Test ProcedureMatrixMethodTAT
Blue-Green Algae ScreenWaterBART (HACH)10 days
Biolgical AssessmentWater, Wipe, Bulk, WipeCulture (Fungi & Bacteria)5 - 7 days
Fungal ProfileWater, Wipe, Bulk, AirCulture / Direct5 - 7 days

CDC   Sampling Procedure for Legionella Testing


Simplified testing for Legionella pneumophila: LegiolertTM

LegiolertTM is a simple and accurate culture test used to detect and quantify Legionella pneumophila, the primary cause of Legionnaires' disease, that provides a confirmed result in 7 days!

"NEW" product developed by IDEXX.

Legionella Culture

ISO Method 11731:2017(E)

Department of General Services - Drinking Water Lab

Commonwealth of Virginia

Division of Consolidated

Laboratory Services

HPE Sample Collection Procedures for Legionella Testing

References (Peer-Reviewed)

Petrisek, R., & Hall, J. (2018) Evaluation of a most probable number method for the enumeration of Legionella pneumophila from North American potable and nonpotable water samples. J. Water & Health 16(1), 25-33. Weblink

Sartory. D.P., Spies, K., Lange, B., Schneider, S., & Langer., B. (2017) Evaluation of a most probable number method for the enumeration of Legionella pneumophila from potable and related water samples. Letters in Applied Microbiology 64(4), 271-275. Article